In vivo and in vitro insulin and fasting control of the transmembrane fatty acid transport proteins in Atlantic salmon (Salmo salar).
نویسندگان
چکیده
We have examined the nutritional and insulin regulation of the mRNA expression of transmembrane fatty acid (FA) transporters [FA transport protein-1 (FATP1) and CD36] together with the lipoprotein lipase (LPL), the cytosolic FA carrier FA binding protein (FABP3), and mitochondrial FA-CoA and -carnitine palmitoyl transferase carriers (CPT)1 and -2 in Atlantic salmon tissues and myocyte cell culture. Two weeks of fasting diminished FATP1, CD36, and LPL in adipose tissue, suggesting a reduction in FA uptake, while FABP3 increased in liver, probably enhancing the transport of FA to the mitochondria. Insulin injection decreased FATP1 and CD36 in white and red muscles, while both transporters were upregulated in the adipose tissue in agreement with the role of insulin-inhibiting muscle FA oxidation and stimulating adipose fat stores. Serum deprivation of 48 h in Atlantic salmon myotubes increased FATP1, FABP3, and CPT-2, while CPT-1 was diminished. In myotubes, insulin induced FATP1 expression but decreased CD36, FABP3, and LPL, suggesting that FATP1 could be more involved in the insulin-stimulated FA uptake. Insulin increased the FA uptake in myotubes mediated, at least in part, through the relocation of FATP1 protein to the plasma membrane. Overall, Atlantic salmon FA transporters are regulated by fasting and insulin on in vivo and in vitro models.
منابع مشابه
In vivo and in vitro insulin and fasting control of the 1 transmembrane fatty acid transport proteins in Atlantic 2 salmon ( Salmo salar ) 3
Departament de Fisiologia, Facultat de Biologia, Universitat de Barcelona. 6 Av. Diagonal 645, E-08028 Barcelona, Catalunya, Spain. 7 Nofima Marin, Norwegian Institute of Food, Fisheries and Aquaculture 8 Research, P.O. Box 5010, NO-1432 Ås, Norway. 9 National Institute of Nutrition and Seafood Research (NIFES), P.O. Box 1
متن کاملEffect of feeding rate on nutrient digestibility in Atlantic salmon, Salmo salar L.
A digestibility trial was conducted to examine the effect of feeding rate on dry matter, gross energy, crude protein and phosphorus digestibility in Atlantic salmon (Salmo salar). Duplicate groups of fish were fed 0.25, 0.5, 0.75, 1.0, 1.25 and 1.9% BW/day. The faeces were collected by Guelph-type collectors for five successive days. Dry matter, protein and phosphorus digestibility’s were all s...
متن کاملHistopathological study of gills in experimentally amoebic gill disease (AGD) infected Atlantic salmon, Salmo salar, L.
Amoebic gill disease (AGD) is the most important parasitic disease of Atlantic salmon industry inAustralia. Atlantic salmon (Salmo salar) experimentally infected with Neoparamoeba sp. apparently showedAGD gross signs on the gill and an amoebic-associated gill pathology. Physico-chemical factors of waterduring the experiment were monitored regularly and were approximately constant (temperature: ...
متن کاملEffect of feeding rate on nutrient digestibility in Atlantic salmon, Salmo salar L.
A digestibility trial was conducted to examine the effect of feeding rate on dry matter, gross energy, crude protein and phosphorus digestibility in Atlantic salmon (Salmo salar). Duplicate groups of fish were fed 0.25, 0.5, 0.75, 1.0, 1.25 and 1.9% BW/day. The faeces were collected by Guelph-type collectors for five successive days. Dry matter, protein and phosphorus digestibility’s wer...
متن کاملNutritional and environmental regulation of highly unsaturated fatty acid synthesis and fatty acid oxidation in Atlantic salmon (Salmo salar L.) enterocytes and hepatocytes
Running title: PUFA metabolism in salmon fed vegetable oil
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- American journal of physiology. Regulatory, integrative and comparative physiology
دوره 301 4 شماره
صفحات -
تاریخ انتشار 2011